Comparative Study between Some Additives on Immune Response of Infectious Bursal Disease Vaccine in Broiler Fed Diet with Aflatoxin-contaminated Poisons

This study was carried out to determine the influence of vit. E with selenium, poultry star, levamezol and acetic acid on immune response to infections bursal disease in broiler fed diet with aflatoxin-contaminated aflatoxin poisons. 200 one-day old chicks were divided randomly into six equal groups, All groups (except 6 group) were fed diet with aflatoxincontaminated feed (from 1 to 40 days old) as following: 1 group was given poultry star, 2 was given vit. E and selenium, 3 group was given levamezol, 4 group was given acetic acid, 5 group only fed aflatoxin (controle positive) and 6 group (control negative). Vit. E, poultry star, levamezol and acetic acid were given by drinking water (from 1 to 25 days old). All groups were vaccinated with IBD vaccine at 12 days old. Blood samples were collected to estimate the immune response by ELISA test. Samples from bursa of fabricius and spleen were taken for histopathological test at 15, 25and 35days old. The results revealed that aflatoxin at level 8ppb was caused significant reduction in Ab titers levels against IBD vaccine in all ages and caused histopathological lesion in lymphoid organs (bursa of fabricious and spleen) which showed marked depletion and reduction of lymophid follicle, while in spleen The lesion was showed depletion, congestion and hemorrhage also the results were showed that adding vit. E and selenium and poultry star lead to significant increasing in Ab titers level against IBD vaccine also reduced the histopathological lesion in bursa of fabricious and spleen which showed slight depletion and hyperplasia of lymphocyte in bursa of fabricius while in spleen showed slight hmg, congestion and slight depletion. It is conclude that aflatoxin in 8ppb in chicken fed had negative effect on IBD vaccination also cause histopathological change in bursa and spleen. On the other hand, using vit. E with selenium and poultry star were reduced the effect of aflatoxin on immune response and protect the lymphoid organs. Whereas, levamesole and acetic acid were act in less degree as compared with vit. E and poultry star concerning the reduction of the effect of aflatoxin in immune response and protection of the lymphoid tissue.